Aim: Optimizing the rate of transcription of genes by applying suitable transciption factors with respect to transcription region of selected gene.

Selected gene: HSPD1; heat shock 60kDa protein 1 (chaperonin)

Transcription site: ONLY the transcription site of the gene is taken, including activator sites, inhibitor sites and promoter region.

Procedures:
1: Transcription site of HSPD1 is isolated
2: promoterless vector is ligated with HSPD1 gene transcription site
3: Re-combinated DNA is inserted into host (E.Coli)
4: Transgenic E.Coli is grown in agar medium and tested with different conditions to determine transcription factors.
5: Varying transcription factors to optimize rate of transcription

Result: Will determine the optimum transcription factors of HSPD1 transcription site and may use transcription in other genes to optimize transcription.

Impacts of research: With the determination of suitable transcription factors to increase or decrease the rate of transcription, one may control the rate of transcription of genes and indirectly controlling the rate of synthesis of protein or et cetera, with respect to the gene.
E.G. Increasing the rate of synthesis of insulin by optimizing rate if transcription of insulin gene. (Commercial use)